Tesco plans to replace 1,700 deputy managers in its chain of Express convenience stores.
Their work will be taken by an extra 3,200 lower paid "shift leaders", increasing staff numbers by 1,500.
The retailer said the deputy managers would be offered the new roles, be redeployed or redundancy payments.
Tesco announced 1,000 job cuts in January as part of its plan to cut the number of its distribution centres from 25 to 23.
The supermarket said: "For any that do make the change from deputy manager to shift leader, we'll be financially supporting them."
Last month, Tesco announced it had agreed to buy the food wholesale group Booker for £3.7bn.
In recent years the UK's big four supermarket groups have faced stiff competition from smaller rivals such as Aldi and Lidl.
This, combined with decreasing demand for weekly shops at large out-of-town stores, has forced them to revise their plans for expansion. Some poorly performing stores have been closed, while plans for new outlets have been abandoned.
Earlier this month, Waitrose said it would shut six supermarkets and also remove a layer of management, cutting 700 jobs in the process, along with the removal of 180 department manager posts in its 350 stores.
For the Christmas period, Tesco said like-for-like sales - which strip out the impact of new store openings - rose 0.7% in the UK, and were up by 0.3% across the whole group.