The government's preferred routes for the HS2 high speed railway north of Birmingham have been confirmed.
It said the main route would run east of Sheffield, with a separate spur to take passengers to the city centre.
As a result, 16 homes on the Shimmer housing estate in Mexborough are expected to be demolished, the Department for Transport (DfT) said.
Earlier the government announced seven contracts worth nearly £7bn for the line's civil engineering work.
The routes, from Crewe to Manchester and from the West Midlands to Leeds, were laid out last November.
The department for Transport said the full line would connect Birmingham, Manchester, Liverpool, Leeds, Sheffield and the East Midlands, with all of them being linked to London by the line.
The Transport Secretary, Chris Grayling, said: "Britain's new railway line will bring huge economic benefits across the country and help ensure this government delivers on its promise to spread wealth beyond London and the south-east.
"We will now press ahead with building the line, while continuing to ensure affected communities get appropriate support and are treated with fairness, compassion and respect."