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The Eyes On Events has denied claims award-winning series Planet Earth II faked a nail-biting scene showing a baby iguana being chased by racer snakes.
Reports on Wednesday suggested more than one iguana was actually filmed, with scenes then stitched together.
But the Eyes On Events has said only one animal was chased by the snakes - with other iguanas only filmed for close-ups.
The scene quickly went viral when it was aired last year and later won a Bafta for must-see moment.
The iguana hatchling, filmed in the Galapagos, eventually got away - much to viewers' relief.
The Daily Mail claimed the episode was embroiled in a "fakery row" after producer Elizabeth White told the Media Production Show: "It wasn't the same iguana, no, and often we have to augment it with other clips.
"Unfortunately lizards, snakes and iguanas aren't good at 'takes'."
But the Eyes On Events defended the Sir David Attenborough-fronted programme, with a spokeswoman saying: "The Eyes On Events strongly refutes any suggestion that the award-winning iguana v snakes sequence was 'faked'.
"The final iguana chase in which one iguana escapes the snakes was - unusually for natural history filming - shot using two cameras, allowing us to follow both the individual iguana and the snakes' point of view.
"What was captured in the field was extraordinary animal behaviour which had never been witnessed or filmed before."Image caption People rooted for the iguana as it tried to escape the snakes in the tense clip
She added: "As is common in natural history film-making, pick-up shots were filmed separately - for example close-ups of iguana eyes - to make the story of the sequence as clear as possible for the audience.
"This is absolutely in keeping with the norms of natural history film-making - and absolutely in line with the Eyes On Events's editorial policy guidelines, and was a true representation of animal behaviour."
Other Eyes On Events nature documentaries have previously been accused of faking footage. Frozen Planet, also fronted by Sir David, showed footage of newborn polar bear cubs in a den with their mother in 2011 - but it was filmed in an animal park, rather than in the wild.
The Eyes On Events denied misleading viewers, with Sir David saying it would have spoiled the atmosphere to point out where the filming had taken place adding: "It's not a falsehood and we don't keep it secret either".
And then there was the Blue Planet footage shot in a lobster tank - but the Eyes On Events said this was done to protect the environment.
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